A team of scientists at NYU Grossman School of Medicine, have found how a key immune signal has a previously unknown role in turning off the immune system’s attack on pancreatic cancer cells.
The study confirmed that interleukin-1β (IL-1β), an immune signaling protein, is made and released by pancreatic tumor cells. This signaling protein was shown to reduce anti-cancer immune responses, which promoted the growth of pancreatic ductal adenocarcinoma or PDA, a form of cancer that is usually deadly within two years.
The results of the study, published online in Cancer Research, a journal of the American Association for Cancer Research, on January 8, 2020, demonstrated that blocking the action of IL-1β in mice with specific immune proteins caused a 32% decrease in PDA tumor growth.
Other experiments combined the anti-IL-1β antibody, which attaches onto and neutralizes its target, with an already approved antibody treatment that shuts down the protein “checkpoint” called PD1.
To spare normal cells from immune attack, the immune system uses “checkpoints” on immune cells that turn them off when they receive the right signal. Cancer cells hijack these checkpoints to turn off the immune system, leading to immune suppression of CD8+ T-cells that would otherwise kill cancer cells.
Therapies called checkpoint inhibitors counter this effect.
However, while effective against many different forms of cancer, checkpoint inhibitors have sofare failed in the treatment of patients with
pancreatic ductal adenocarcinoma, with the response rate in tumors as low as roughly three percent in some trials. The limitations were attributed to poor CD8+ T cell infiltration and immune suppression.
In the current study, adding anti-IL-1β antibodies to anti-PD-1 antibody treatment doubled the infiltration of such T-cells into PDA tumors and increased the anti-tumor activity of PD-1 blockade by 40%.
“By engineering mice with versions of PDA that lack the IL-1β gene, we found for the first time that pancreatic cancer cells produce IL-1β, and that it is essential for the continued growth of PDA tumors,” explained the study’s senior author Dafna Bar-Sagi, Ph.D, Vice Dean for Science and Chief Science Officer for NYU Langone Health.
“Blocking IL-1β with an antibody treatment may represent another way to make pancreatic tumors vulnerable to the immune system, with the potential to significantly increase the effectiveness of checkpoint inhibitors if combined,” Bar-Sagi added.
The new finding is in line with past work in other labs that had described the microbiome, the mix of bacterial species in the pancreas, as being altered in the presence of PDA, and a factor in cancer growth.
The production of IL-1β was traditionally contributed to immune cells, but this study demonstrated that pancreatic tumor cells can also make this immune signaling protein in response to proteins given off by certain bacteria.
Bacterial products were found to activate proteins on the cancer cell surfaces called toll-like receptors, which set off chain reactions that were required for IL-1β production in cancer cells.
The research team also found that higher IL-1β production caused nearby pancreatic stellate cells to increase production of dense, structural proteins like collagen. Desmoplasia is the overgrowth of such fibrous tissue that often occurs near pancreatic tumors, and which has been linked to treatment resistance.
Active stellate cells were also found to trigger production of the signaling proteins that attract immune cells called macrophages into tumors and programs them to become the type (M2) that suppresses immune reactions. Experiments also confirmed that higher IL-1β and M2 macrophage levels, along with fibroblast-driven desmoplasia, reduced the ability of cancer-cell-killing CD8+ T cells to enter tumors.
“This work provides strong evidence that blocking the action of IL-1β enables T cells to better penetrate tumors and kill cancer cells, mechanisms with potential to overcome the limitation of currently available immunotherapies in the treatment of pancreatic cancer,” noted the study’s first author Shipra Das, who was a member of Bar-Sagi’s lab at the time the study was done.
Along with Bar-Sagi and Das, study authors from the Department of Biochemistry and Molecular Pharmacology at NYU Grossman School of Medicine were Beny Shapiro, Emily Vucic, and Sandra Vogt. Das, who was a postdoctoral fellow, has since left NYU Langone and continues her work in immune oncology in the biopharmaceutical industry.
While the study authors did not received financial compensation, Novartis provided the mouse anti-PD1 and anti-IL-1β antibodies for this study. The study was further supported by the National Institutes of Health and the Lustgarten Foundation Pancreatic Cancer Convergence Dream Team, Stand Up To Cancer, the Entertainment Industry Foundation, American Association for Cancer Research, and the Canadian Institutes of Health Research.
 Das S, Shapiro B, Vucic EA, Vogt S, Bar-Sagi D. Tumor Cell-Derived IL-1β Promotes Desmoplasia and Immune Suppression in Pancreatic Cancer. Cancer Res January 8 2020 DOI: 10.1158/0008-5472.CAN-19-2080 [Article]