Researchers at Okayama University employed a 3D nano-matrix to gain insights into how different cells types mimic the properties of cancer stem cells in this environment. Their results published in the journal PLoS One shows that a nano-environment promotes distinct patterns of cell aggregation and biological properties that are reminiscent of tumors.[1]

In biological experiments cells are usually grown on a two-dimensional matrices. Although cells stick well to such surfaces, this process of adhesion can sometimes mask the natural biological properties of cells. So there is demand for alternatives to 2D matrix environments to cultivate and study cancer cells.

NanoCulture Plates (NCP) are specialized nano-scale matrices, resembling the scaffolding on a building. These NCPs give cells space to migrate freely without adhering to the bottom and aggregation with each other. All of these properties are essential for tumors.

Favorable properties
Takanori Eguchi, Chiharu Sogawa and colleagues at Okayama University adopted these favorable properties of NCPs to study the properties of 67 biological cell lines, focusing on the range of cells that could successfully transform into cancerous cells. A remarkable feature of cancer cells is their ability to grow in the body even under conditions of low oxygen. Under these conditions, cancer cells tend to start showing properties of stem cells, thus forming ?cancer stem cells? (CSC). The milieu inside cell aggregates can also result in oxygen deprivation. The authors were thus also interested in seeing if CSCs could be induced in NCPs. To sustain the induction of ?stemness?, a growth medium containing enhanced nutrients was used, termed a stem cell inducing medium.


Grape-like aggregation
The researchers observed that while most of the cell lines formed ?spheroids? characteristic of tumor cells, a novel pattern of ?grape-like aggregation (GLA)? was also prominent in some cases. To characterize GLA forming cells further, cells known as PC-3 cells were grown on NCPs and then injected into mice. Not only did PC-3 GLAs enlarge into tumors, but they also rapidly migrated to other organs just like cancer cells, while the spheroids did not show such behavior. The presence of the stem cell inducing medium in NCPs further favored the formation of larger aggregates of PC-3 cells, with very strong intercellular adhesion forces, reminiscent of CSCs.

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Photo 1.0: Representative morphologies of the various types of cell aggregates formed on NanoCulture Plates (NCP). (A) Grape-like aggregation (GLA) of PC-3 cells formed in F12K medium with 10% FBS. (B) Spheroids of CAL 27 cells formed in DMEM with 10% FBS.

Unusual genetic profile
Cancer stem cells or CSCs have an unusual genetic profile, comprising stem cell genes and cancer cell genes. Incubation of PC-3 cells in NCPs resulted in induction of a high number of pluripotency or ?stemness? inducing genes, along with some tumor inducing genes. CSCs also secret intracellular sacs called exosomes, which contain EpCAM- one of CSC proteins. By studying the extracellular fluid, the researchers found that exosome-free HSP90? was also abundantly released under these conditions.

In the study, NCPs were seen to be an excellent mactices for replicating in-vitro tumor synthesis.

Eguchi, Sogawa and colleagues anticipate that, ?These features of NCPs may be useful for advanced tumor and stem cell biology and preclinical testing of novel therapeutics.?

Last Editorial Review: March 20, 2018

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