For hard-to-treat leukemias, lymphomas, and other hematological malignancies, hematopoietic stem cell transplantation (HSCT) is the gold standard of care. The procedure involves replacing a patient’s own blood-forming stem cells with a donor’s stem cells and, in the process, eradicating cancer cells in the blood, lymph nodes, and bone marrow.

However, many patients diagnosed with hematological malignancies are often too fragile to undergo HSCT. One of the primary reasons is that in order to receive a hematopoietic stem cell transplantation, a patient’s stem cells must first be destroyed by intensive chemotherapy and sometimes total body radiation before a donor’s stem cells are infused.

Patient preparation or conditioning regimen makes space for incoming donor stem cells, helps to remove cancer cells remaining in the body, and depletes the patient’s immune system so it can’t attack the donor’s stem cells. However, regimen-related mortality and morbidities, as a result of toxicities and the suppression of the immune system caused by conditioning regimens, put patients at high risk of infections, organ damage, infertility, secondary malignancies, and other life-threatening side effects. This limits the use of HSCT in both malignant and non-malignant conditions.

A new method
Studying mice, researchers at Washington University School of Medicine in St. Louis have, for the first time, developed a method of HSCT that does not require radiation or chemotherapy as part of patient conditioning.

Instead, the conditioning strategy takes an immunotherapeutic approach, combining the targeted elimination of blood-forming stem cells in the bone marrow with immune-modulating drugs to prevent the immune system from rejecting the new donor stem cells.[1]

Advertisement #3

With the new technique, mice underwent successful hematopoietic stem cell transplantation from unrelated mice without evidence of dangerously low blood cell counts that are a hallmark of the traditional procedure. The data also suggested that such stem cell transplants can be effective against leukemia.

The study, supported by the National Cancer Institute (NCI) Outstanding Investigator Award of the National Institutes of Health (NIH) and others, was published in the online first edition of the Journal of Clinical Investigation and opens the door to safer stem cell transplantation. If successful the result may be that more patients with various types of hematological malignancies could receive this potentially curative therapy, and it could be considered as a treatment for other diseases, such as sickle cell anemia or other genetic disorders, that are less life-threatening.[1]

John F. DiPersio, MD, Ph.D., the Virginia E. & Sam J. Golman Professor of Medicine and chief of the Division of Oncology at Washington University School of Medicine.

“To be able to do a stem cell transplant without having to give radiation or chemotherapy would be transformative,” said senior author and medical oncologist John F. DiPersio, MD, Ph.D., the Virginia E. & Sam J. Golman Professor of Medicine and chief of the Division of Oncology at Washington University School of Medicine.

“It could eliminate the dangerously low blood cell counts, bleeding complications, organ damage, and infections. It has particular implications for conducting bone marrow transplantation or gene therapy for patients with noncancerous diseases such as sickle cell anemia, where the toxicities of chemotherapy- or radiation-associated conditioning are important to avoid. We have more work to do before we’re ready to translate these findings to people, but we’re encouraged by the results of this study,” DiPersio explained.

Antibody-drug conjugates
As an alternative to high-dose chemotherapy and whole-body radiation, DiPersio, who also directs the Center for Gene & Cellular Immunotherapy, and his colleagues harnessed drugs that are toxic to cells, and attached these drugs to CD45- or cKit-targeted antibodies that target specific surface proteins that are expressed primarily on bone marrow stem cells. Only when these antibody-drug conjugates (ADCs) bind to those specific proteins are they then internalized by the stem cells, which leads to the release of the drug payload inside the cell and, ultimately, cell death. Using the ricin derivative saporin, a so-called ribosome inactivating protein or RIP, as the drug payload, the researchers generated two different ADCs to target two specific proteins found on the surface of blood stem cells, which minimizes the potential for them to cause damage to other cell types.

To prevent the recipient’s immune system from then rejecting the donor cells, the researchers treated the mice with immunosuppressive compounds called Janus kinase (JAK) inhibitors. In this study, the investigators primarily used baricitinib (Olumiant®; Eli Lilly and Company), a drug approved by the U.S. Food and Drug Administration used to treat adults with moderately to severely active rheumatoid arthritis (RA) who have tried at least one other medicine called a tumor necrosis factor (TNF) antagonist that did not work well enough or could not be tolerated. They found that baricitinib prevented the recipient’s immune cells, including T cells and natural killer cells, from attacking the donor stem cells.

The researchers found that ADCs provide the same benefits of full-intensity conditioning to remove disease-causing cells that minimize toxicity, reducing the severity of treatment-related adverse events while retaining efficacy.

“By combining the antibody-drug conjugates with JAK inhibitors, we were able to achieve a successful transplant between two completely unrelated strains of mice,” noted first author Stephen P. Persaud, MD, Ph.D., an instructor in pathology & immunology.

“A successful transplant across such a stringent immunological barrier is promising for eventually being able to harness this technique for patients with leukemia,” Persaud added.

Initial studies using ADC as conditioning agents have largely focused on syngeneic HSCT. However, to treat acute leukemias or induce tolerance for solid organ transplantation, this approach must be expanded to allogeneic HSCT (allo-HSCT).

Striking a balance
The researchers also found that the new technique struck a balance between the donor immune cells attacking leukemia cells — called the graft-versus-leukemia effect — in a common mouse model of leukemia and not attacking the recipient’s healthy tissues, a dangerous condition called graft-versus-host disease. The mice in this study did not develop graft-versus-host disease because the immune-suppressing drugs prevented it, another unique and significant advantage of this approach, according to the researchers.

“When you give JAK inhibitors from the beginning, there is evidence that they prevent graft-versus-host disease from developing later,” DiPersio explained, who serves as deputy director of Siteman Cancer Center at Barnes-Jewish Hospital and Washington University School of Medicine.

“In addition, unlike a regular transplant using radiation and chemotherapy as transplant conditioning, none of the mice developed any reduction in their blood counts, which is the major life-threatening complication of traditional stem cell transplants. Chemotherapy and radiation destroy all the old cells at once. With the new strategy, the old cells were slowly replaced by donor cells, and so we never saw any drop in the blood cell counts in these mice. The blood cell counts looked normal the whole way, and in the end, we could see that all the blood cells originated from the new donor cells.”

After a period of time, the researchers found that they could gradually reduce the JAK inhibitors and, once the donor stem cells totally replaced the original cells, stop the immune suppression altogether.

“We’ve shown that we can use this relatively simple regimen that is minimally toxic to transplant donor stem cells across immunologic barriers in mice,” Persaud said.

“We need more research to see if the same strategy will be applicable to humans. We are working to optimize the technique in mice, and then we will likely test it in other animal models of leukemia before we would begin planning a clinical trial to investigate the strategy in patients,” Persaud concluded.

Patent application
Working with Washington University’s Office of Technology Management, DiPersio and Persaud have filed a patent application for the combination of antibody-drug conjugates with JAK inhibitors for transplant conditioning.

Highlights of prescribing information
Baricitinib (Olumiant®; Eli Lilly and Company) (Prescribing Information)

[1] Persaud SP, Ritchey JK, Kim S, Lim S, Ruminski PG, Cooper ML, Rettig MP, Choi J, DiPersio JF. Antibody-drug conjugates plus Janus kinase inhibitors enable MHC-mismatched allogeneic hematopoietic stem cell transplantation. J Clin Invest. 2021 Nov 2:e145501. doi: 10.1172/JCI145501. Epub ahead of print. PMID: 34730109.[Article]

Featured Image: Comforting a Patient. Courtesy: © 2016 – 2021 Fotolia/Adobe. Used with permission.

This article was first published in ADC Review | Journal of Antibody-drug Conjugates on November 12, 2021.

Advertisement #5